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OmpA-like protein influences cell shape and adhesive activity of Tannerella forsythia.
- Source :
- Molecular Oral Microbiology; Dec2011, Vol. 26 Issue 6, p374-387, 14p, 4 Black and White Photographs, 1 Diagram, 3 Charts, 1 Graph
- Publication Year :
- 2011
-
Abstract
- Summary Tannerella forsythia, a gram-negative fusiform rod, is implicated in several types of oral anaerobic infections. Most gram-negative bacteria have OmpA-like proteins that are homologous to the OmpA protein in Escherichia coli. We identified an OmpA-like protein in T. forsythia encoded by the tf1331 gene as one of the major proteins by mass spectrometric analysis. Two-dimensional, diagonal electrophoresis showed that the OmpA-like protein formed a dimeric or trimeric structure via intermolecular disulfide bonds. A biotin labeling experiment revealed that a portion of the protein was exposed on the cell surface, even though T. forsythia possesses an S-layer at the outermost cell surface. Using a tf1331-deletion mutant, we showed that the OmpA-like protein affected cell morphology. The length of the mutant cell was reduced almost by half. Cell swelling was observed in more than 40% of the mutant cells. Moreover, the mutant exhibited decreased adhesion to fibronectin, retarded autoaggregation, and reduced cell surface hydrophobicity. These results suggest that the OmpA-like protein in T. forsythia plays an important role in cellular integrity and adhesive function. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 20411006
- Volume :
- 26
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Molecular Oral Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 67036437
- Full Text :
- https://doi.org/10.1111/j.2041-1014.2011.00625.x