Electrochemical mechanism of rusticyanin (Rus.) isolated from A. ferrooxidans measured by Rus.-ZnS-QDs/L-Cys/Au electrode.

Electrochimcal behaviors of rusticyanin (Rus.) isolated from Acidithiobacillus ferrooxidans were investigated through Rus.-ZnS-QDs/L-Cys/Au electrode. The cyclic voltammetric results indicate that rusticyanin immobilized on the surface of Rus.-ZnS-QDs/L-Cys/Au electrode can undergo a direct quasi-reversible electrochemical reaction. The immobilized rusticyanin is not denatured and still retains its activity in the temperature range of 19-43 °C. The reduction ability of the protein increases and its oxidation ability becomes weak with the increase of pH from 6.0 to 7.8. Fe ions in the solution can promote the electron transfer kinetics of the immobilized rusticyanin and make its peak potentials ( φ) markedly move negatively. [ABSTRACT FROM AUTHOR]