Effect of P38 MAPK on the apoptosis of human lung adenocarcinoma cell induced by the spider venom.

Background: The venom of spiders may inspire new drugs to treat carcinoma. The aim of this study was to investigate the effect of P38 MAPK on tumor necrosis factor-related apoptosis of lung adenocarcinoma cell line by spider venom. Methods: Lung adenocarcinoma A549 cells were cultured in PRMI-1640 medium and the apoptosis rate was observed after treatment with spider venom with a flow cytometer. The expression of P38 MAPK protein in lung cancer cells was analyzed using Western blot. Results: After targeting spider venom intervention, the expression of P38 MAPK in the cells compared to the control group was significantly down-regulated. The anticancer potency of spider venom is associated with decreased expression of P38 MAPK of lung adenocarcinoma cancer cells. [ABSTRACT FROM AUTHOR]