Direct Isolation of Genomic DNA from Marine Soil Sediments Followed By Purification for Construction of Metagenomics Library.

Objectives: To assess the genomic DNA from marine soil sediment for construction metagenomic library. Methods: The soil samples were collected from the study area at coastal regions of Samiyarpettai, Cuddalore District, TamilNadu,(south India),during the month of April 2010. Genomic DNA was extracted for marine soil sediment by the method of direct isolation (nature protocol), purification of DNA is followed by the chromous gel extraction kits. Results: Approximately 6-7 mg of genomic DNA extract was derived for construction of metagenomic library, and also high quality DNA was derived by the elctroelution purification process. Conclusion: From this study it is confirmed that the direct isolation of DNA method is an efficient protocol for isolation of high amount and good quality of genomic DNA for the construction of metagenomic library. The elctroelution (Chromous Gel Extraction kits) method is an effective for purifying the genomic DNA and also to get rid off humic substances and other contaminants when compare to various purification kits. [ABSTRACT FROM AUTHOR]