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Regulated expression of the ubiquitin protein ligase, E3Histone/LASU1/Mule/ARF-BP1/HUWE1, during spermatogenesis.
- Source :
- Developmental Dynamics; Oct2007, Vol. 236 Issue 10, p2889-2898, 10p
- Publication Year :
- 2007
-
Abstract
- A ubiquitin protein ligase (E3), E3<superscript>Histone</superscript>/LASU1 (Mule/ARF-BP1/HUWE1), was recently identified that mediates ubiquitination of core histones, the Mcl-1 anti-apoptotic protein, and the p53 tumor suppressor protein. However, the expression of E3<superscript>Histone</superscript>/LASU1 remains poorly studied. Because we identified E3<superscript>Histone</superscript>/LASU1 from the testis, we explored its regulation during spermatogenesis. In the first wave of rat spermatogenesis, E3<superscript>Histone</superscript>/LASU1 mRNA and protein had peak expression at days 10 and 20, respectively, and decreased with age. Consistent with these findings, immunohistochemistry revealed that E3<superscript>Histone</superscript>/LASU1 was highly expressed in nuclei from spermatogonia to mid-pachytene spermatocytes. There was no obvious staining in spermatids, when histones are ubiquitinated and degraded. E3<superscript>Histone</superscript>/LASU1 was also expressed in other tissues. However, except in neuronal cells of the brain, expression was cytoplasmic. Thus, E3<superscript>Histone</superscript>/LASU1 may play a role in chromatin modification in early germ cells of the testis, but also has functions in other tissues. Developmental Dynamics 236:2889-2898, 2007. © 2007 Wiley-Liss, Inc. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10588388
- Volume :
- 236
- Issue :
- 10
- Database :
- Complementary Index
- Journal :
- Developmental Dynamics
- Publication Type :
- Academic Journal
- Accession number :
- 63327663
- Full Text :
- https://doi.org/10.1002/dvdy.21302