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Enhancement of bacteriolysis of Shuffled phage PhiX174 gene E.
- Source :
- Virology Journal; 2011, Vol. 8 Issue 1, p206-211, 6p
- Publication Year :
- 2011
-
Abstract
- Bacterial ghosts that are generated using the regulated PhiX174 lysis gene E offer a new avenue for the study of inactivated vaccines. Here, we constructed a library of mutant gene E using a gene-shuffling technique. After screening and recombination with the prokaryotic non-fusion expression vector pBV220, two lysis plasmids were selected. Among which, a novel mutant E gene (named mE), consisting of a 74-bp non-encoding sequence at 5'-end and a 201-bp gene ΔE, significantly increased the lysis effect on prokaryotic Escherichia coli and Salmonella enteritidis. Moreover, lysis efficiency, as measured by the OD<subscript>600</subscript> value, reached 1.0 (10<superscript>9</superscript> CFU), avoiding the bottleneck problem observed with other bacterial lysis procedures, which results in a low concentration of bacteria in suspension, and consequent low production of bacterial ghosts. Our results may provide a promising avenue for the development of bacterial ghost vaccines. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 1743422X
- Volume :
- 8
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Virology Journal
- Publication Type :
- Academic Journal
- Accession number :
- 62806493
- Full Text :
- https://doi.org/10.1186/1743-422X-8-206