Effect of culture conditions on shoot regeneration of hypocotyl explants from Perilla frutescens.

Culture conditions affecting regeneration of hypocotyls from Perilla frutescens were investigated. The results showed that seedling age, preculture time in darkness and hormone concentrations had important influences on regeneration ratio of shoots. The highest ratio of regeneration was realized when the hypocotyls of 13 - days seedling were precultured for 8 days in darkness and then cultured under the condition of 14h/d light in the medium of MS + 3.0mg/L BA + 0.3mg/L NAA. The optimal medium for inducing root from regeneration shoot was 1/2MS + 1.0mg/L BA +0. 3mg/L IAA. [ABSTRACT FROM AUTHOR]