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Successful extraction of DNA from archived alcohol-fixed white-eye fish specimens using an ancient DNA protocol.

Authors :
De Bruyn, M.
Parenti, L. R.
Carvalho, G. R.
Source :
Journal of Fish Biology; Jun2011, Vol. 78 Issue 7, p2074-2079, 6p
Publication Year :
2011

Abstract

A protocol used routinely for rapid ancient DNA extraction was applied to fish tissue archived over 80 years ago. The method proved successful, whereas other extraction protocols failed. Researchers working on DNA from older archived fish samples are encouraged to continue to concentrate their efforts on 'white-eye' specimens, which indicate an alcohol-based fixative and are thus likely to yield viable DNA. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00221112
Volume :
78
Issue :
7
Database :
Complementary Index
Journal :
Journal of Fish Biology
Publication Type :
Academic Journal
Accession number :
61140198
Full Text :
https://doi.org/10.1111/j.1095-8649.2011.02975.x