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Endogenously released GLP-1 is not sufficient to alter postprandial glucose regulation in the dog.

Authors :
Johnson, Kathryn M. S.
Farmer, Tiffany
Schurr, Kathleen
Donahue, E. Patrick
Farmer, Ben
Neal, Doss
Cherrington, Alan D.
Source :
Endocrine (1355008X); Jun2011, Vol. 39 Issue 3, p229-234, 6p, 5 Graphs
Publication Year :
2011

Abstract

Glucagon-like peptide-1 (GLP-1) is secreted from the L cell of the gut in response to oral nutrient delivery. To determine if endogenously released GLP-1 contributes to the incretin effect and postprandial glucose regulation, conscious dogs ( n = 8) underwent an acclimation period ( t = −60 to −20 min), followed by a basal sampling period ( t = −20 to 0 min) and an experimental period ( t = 0-320 min). At the beginning of the experimental period, t = 0 min, a peripheral infusion of either saline or GLP-1 receptor (GLP-1R) antagonist, exendin (9-39) (Ex-9, 500 pmol/kg/min), was started. At t = 30 min, animals consumed a liquid mixed meal, spiked with acetaminophen. All animals were studied twice (± Ex-9) in random fashion, and the experiments were separated by a 1-2-week washout period. Antagonism of the GLP-1R did not have an effect, as indicated by repeated-measures MANOVA analysis of the Δ AUC from t = 45-320 min of arterial plasma glucose, GLP-1, insulin, glucagon, and acetaminophen levels. Therefore, endogenous GLP-1 is not sufficient to alter postprandial glucose regulation in the dog. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
1355008X
Volume :
39
Issue :
3
Database :
Complementary Index
Journal :
Endocrine (1355008X)
Publication Type :
Academic Journal
Accession number :
60465746
Full Text :
https://doi.org/10.1007/s12020-011-9441-x