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Construction of an Escherichia coli mutant producing monophosphoryl lipid A.
- Source :
- Biotechnology Letters; May2011, Vol. 33 Issue 5, p1013-1019, 7p
- Publication Year :
- 2011
-
Abstract
- Lipid A is a major component in the outer membrane of most Gram-negative bacteria. Monophosphoryl lipid A contains no phosphate group at 1-position and can be used as an adjuvant. We constructed an Escherichia coli mutant CW001 by integrating a gene lpxE into the chromosome of E. coli W3110. The gene lpxE encodes an enzyme LpxE which removes the 1-phosphate group of lipid A. CW001 predominantly produces 1-dephosphorylated lipid A in vivo, as adjudged by thin layer chromatography and electro-spray ionization mass spectrometry. This study not only is important for the development of lipid A adjuvants but also provides a novel method for integration of heterologous genes into the chromosome of E. coli. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01415492
- Volume :
- 33
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Biotechnology Letters
- Publication Type :
- Academic Journal
- Accession number :
- 60174844
- Full Text :
- https://doi.org/10.1007/s10529-011-0521-z