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High- and low-frequency mechanical properties of living starfish oocytes.
- Source :
- Journal of Biophotonics; May2011, Vol. 4 Issue 5, p324-334, 11p
- Publication Year :
- 2011
-
Abstract
- We studied the mechanical properties of living starfish oocytes belonging to two species, Astropecten Auranciacus and Asterina pectinifera, over a wide range of timescales. We monitored the Brownian motion of microspheres injected in the cytoplasm using laser particle-tracking (LPT) and video multiple-particle-tracking (MPT) techniques, to explore high- and low-frequency response ranges, respectively. The analysis of the mean-square-displacements (MSD) allowed us to characterize the samples on different timescales. The MSD behavior is explained by three power-law exponents: for short times ( τ < 1 ms) it reflects the semiflexible behavior of the actin network; for intermediate timescales (1 ms < τ < 1 s) it is similar to that of a soft-glass material; finally for long times ( τ > 1 s) it behaves mainly like a viscous medium. We computed and compared the viscoelastic moduli using a recently proposed model describing the frequency response of the cell material. The large fluctuations found in the MSD over hundreds of trajectories indicate and confirm the significant cytoplasm heterogeneity. (© 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 1864063X
- Volume :
- 4
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Journal of Biophotonics
- Publication Type :
- Academic Journal
- Accession number :
- 60109609
- Full Text :
- https://doi.org/10.1002/jbio.201000076