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Human pancreatic islets produce and secrete MCP-1/CCL2: relevance in human islet transplantation.
- Source :
- Diabetes; Jan2002, Vol. 51 Issue 1, p55-65, 11p, 1 Graph
- Publication Year :
- 2002
-
Abstract
- We investigated the capacity of human islets to produce monocyte chemoattractant protein-1 (MCP-1). Primary cultures of pancreatic islets expressed and secreted MCP-1, as determined by Northern blot, immunohistochemistry, in situ hybridization, and enzyme-linked immunosorbent assay. The produced MCP-1 was biologically active as it attracted monocytes in chemotaxis assay, and chemotactic activity was almost abrogated by a neutralizing anti-MCP-1 monoclonal antibody. Expression of MCP-1 was increased by primary inflammatory cytokines (interleukin-1 beta, tumor necrosis factor-alpha) and lipopolysaccharide at both the mRNA and protein levels but not by glucose. However, MCP-1 did not modulate insulin secretion. MCP-1 secreted by pancreatic islets plays a relevant role in the clinical outcome of islet transplant in patients with type 1 diabetes. In fact, low MCP-1 secretion resulted as the most relevant factor for long-lasting insulin independence. This finding opens new approaches in the management of human islet transplantation. Finally, the finding that MCP-1 appears constitutively present in normal human islet beta-cells (immunohistochemistry and in situ hybridization), in the absence of an inflammatory infiltrate, suggests that this chemokine could have functions other than monocyte recruitment and opens a new link between the endocrine and immune systems. [ABSTRACT FROM AUTHOR]
- Subjects :
- ISLANDS of Langerhans
MONOCYTES
SECRETION
Subjects
Details
- Language :
- English
- ISSN :
- 00121797
- Volume :
- 51
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Diabetes
- Publication Type :
- Academic Journal
- Accession number :
- 5839147
- Full Text :
- https://doi.org/10.2337/diabetes.51.1.55