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Antioxidant activity and free radical-scavenging capacity of Gynura divaricata leaf extracts at different temperatures.

Authors :
Chunpeng Wan
Yanying Yu
Shouran Zhou
Wei Liu
Shuge Tian
Shuwen Cao
Source :
Pharmacognosy Magazine; 2011, Vol. 7 Issue 25, p40-45, 6p
Publication Year :
2011

Abstract

Background: Extraction temperature influences the total phenolic content (TPC), total flavonoid content (TFC) of medicinal plant extracts to a great extend. TPC and TFC are the principle activity constituents present in the plant. The effects of extraction temperature on TPC, TFC and free radical-scavenging capacity of Gynura divaricata leaf extracts are worth to study. Materials and Methods: Folin-Ciocalteu and aluminum chloride colorimetric assay were used to determine the TPC and TFC of Gynura divaricata leaf extracts at different temperatures. The antioxidant and free radical-scavenging activity were measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azinobis-( 3-ethylbenzthiazoline-6-sulfonic acid (ABTS) and phosphomolybdenum methods. Results: TPC and TFC were significantly elevated with increasing extraction temperature (from 40°C to 100°C). However, TPC and TFC were not significantly different (P > 0.05) at the extraction temperatures 90°C and 100°C. Also, the extracts obtained at a higher temperature exhibited a significant free radical-scavenging activity compared with extraction at lower temperatures (P < 0.05). The TPCs (13.95-36.68 mg gallic acid equivalent/g dry material) were highly correlated with DPPH (R² = 0.9229), ABTS (R² = 0.9951) free radical-scavenging capacity, and total antioxidant activity (R² = 0.9872) evaluated by phosphomolybdenum method. Conclusion: The TPC and TFC of G. divaricata leaf was significantly influenced by the extraction temperatures, which were the main antioxidant constituents present in the G. divaricata plant. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09731296
Volume :
7
Issue :
25
Database :
Complementary Index
Journal :
Pharmacognosy Magazine
Publication Type :
Academic Journal
Accession number :
57632401
Full Text :
https://doi.org/10.4103/0973-1296.75900