Cell-free synthesis of proteins that require disulfide bonds using glucose as an energy source.

The article discusses a study which created a cell-free protein synthesis extract which produces disulfide-bond requiring proteins and having glucose as energy source. It is noted that cell-free synthesis (CFPS) can be used to replace bacterial fermentation in the production of recombinant proteins. The study concluded that the CFPS reactions with KGK10 extracts produce UK and mGM-CSF proteins which require disulfide bonds.