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Aspirin Increases Apolipoprotein-A-I-Mediated Cholesterol Efflux via Enhancing Expression of ATP-Binding Cassette Transporter A1.
- Source :
- Pharmacology; 2010, Vol. 86 Issue 5/6, p320-326, 7p
- Publication Year :
- 2010
-
Abstract
- Background: The efflux of cellular cholesterol mediated by apolipoprotein (apo)A-I and ATP-binding cassette transporter A1 (ABCA1) is a major pathway of reverse cholesterol transport. We investigated the effect of aspirin on this process. Methods: The expression levels of ABCA1 in RAW264.7 cells were determined using reverse transcription polymerase chain reaction and Western blot analysis. <superscript>3</superscript>H-cholesterol efflux was measured by scintillation counting. Results: 0.5 mmol/l aspirin increased apoA-I-mediated cholesterol efflux and increased the expression of ABCA1. By increasing the dose of aspirin higher than 0.5 mmol/l, ABCA1 expression and function were significantly decreased. In cells transfected with a specific peroxisome proliferator-activated receptor (PPAR)-α small interfering RNA, the induction of ABCA1 expression and apoA-I-mediated <superscript>3</superscript>H-cholesterol efflux by aspirin were substantially suppressed. Conclusions: The data demonstrate that low-dose aspirin increases ABCA1 expression via a PPAR-α-dependent mechanism and increases apoA-I-mediated cholesterol efflux. Copyright © 2010 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00317012
- Volume :
- 86
- Issue :
- 5/6
- Database :
- Complementary Index
- Journal :
- Pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 56945332
- Full Text :
- https://doi.org/10.1159/000321727