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Artificial insemination of single women and lesbian women with donor semen: Recycling the single cell to detect specific chromosomes and to investigate specific gene sequences.
- Source :
- Human Reproduction; Nov1994, Vol. 9 Issue 11, p2150-2155, 6p
- Publication Year :
- 1994
-
Abstract
- We have developed a new procedure, called cell recycling, which combines the two powerful techniques of polymerase chain reaction (PCR) and fluorescent in-situ hybridization (FISH) on the same single cell. A fixed cell is used as the DNA template for PCR, prior to the FISH analysis. Using single blastomeres from mouse embryos as a model system, cell recycling procedures detect the single-copy β-haemoglobin gene sequence at an efficiency of 70% as well as sex chromosome constitution at an efficiency of 74% in the same single cell. Cell recycling will increase the success rate of pregnancy following preimplantation diagnosis for a specific gene defect by identifying embryos with chromosomal abnormalities and eliminating them from the transfer procedure. [ABSTRACT FROM PUBLISHER]
Details
- Language :
- English
- ISSN :
- 02681161
- Volume :
- 9
- Issue :
- 11
- Database :
- Complementary Index
- Journal :
- Human Reproduction
- Publication Type :
- Academic Journal
- Accession number :
- 55966156