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A self-powered, one-step chip for rapid, quantitative and multiplexed detection of proteins from pinpricks of whole bloodElectronic supplementary information (ESI) available: Figures S1–S7, Tables S1 and S2, Videos S1–S6, and Methods. See DOI: 10.1039/c0lc00132e

Authors :
Jun Wang
Habib Ahmad
Chao Ma
Qihui Shi
Ophir Vermesh
Udi Vermesh
James Heath
Source :
Lab on a Chip; Oct2010, Vol. 10 Issue 21, p3157-3162, 6p
Publication Year :
2010

Abstract

We describe an automated, self-powered chip based on lateral flow immunoassay for rapid, quantitative, and multiplex protein detection from pinpricks of whole blood. The device incorporates on-chip purification of blood plasma by employing inertial forces to focus blood cells away from the assay surface, where plasma proteins are captured and detected on antibody “barcode” arrays. Power is supplied from the capillary action of a piece of adsorbent paper, and sequentially drives, over a 40 minute period, the four steps required to capture serum proteins and then develop a multiplex immunoassay. An 11 protein panel is assayed from whole blood, with high sensitivity and high reproducibility. This inexpensive, self-contained, and easy to operate chip provides a useful platform for point-of-care diagnoses, particularly in resource-limited settings. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
14730197
Volume :
10
Issue :
21
Database :
Complementary Index
Journal :
Lab on a Chip
Publication Type :
Academic Journal
Accession number :
54843903
Full Text :
https://doi.org/10.1039/c0lc00132e