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Highly selective resonance scattering detection of trace thrombin using aptamer-modified AuRe nanoprobe.
- Source :
- Bioprocess & Biosystems Engineering; Nov2010, Vol. 33 Issue 9, p1087-1094, 8p
- Publication Year :
- 2010
-
Abstract
- The gold-rhenium (AuRe) composite nanoparticle was prepared by NaBH reduction procedure, and was modified by the aptamer to obtain an AuRe nanoprobe (AuRessDNA) for thrombin. In pH 7.0 Tris-HCl buffer solution and in the presence of salt, the nanoprobe specifically combined with thrombin to form AuRe-aptamer-thrombin cluster that resulted in the resonance scattering intensity ( I) increasing at 560 nm. The increased intensity Δ I was linear to the thrombin concentration in the range of 0.115-6.93 nmol/L, with a regression equation of Δ I = 53.0 C + 2.5, a correlation coefficient of 0.9989, and a detection limit of 13 pmol/L. This method was applied to detect thrombin in human plasma samples, with satisfactory results. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 16157591
- Volume :
- 33
- Issue :
- 9
- Database :
- Complementary Index
- Journal :
- Bioprocess & Biosystems Engineering
- Publication Type :
- Academic Journal
- Accession number :
- 54409264
- Full Text :
- https://doi.org/10.1007/s00449-010-0434-6