Determination of Cinepazide Maleate in Rat Plasma by LLE Using LC-MS-MS.

liquid chromatography tandem mass spectrometry method employing electrospray ionization has been developed for the determination of cinepazide maleate in rat plasma using alprazolam as the internal standard. Liquid-liquid extraction was used for sample preparation and the analysis was achieved on a C18 reversed phase column. The method was validated over the concentration range 1-2,000 ng mL in rat plasma. The intra-day and inter-day precision of cinepazide maleate expressed as RSD < 10% and the relative error (RE) did not exceed 10%. This validated method is a novel technique for sample preparation and quantitation, which was successfully applied to pharmacokinetic studies of cinepazide maleate in rats following a single oral administration dose of 10 mg kg. [ABSTRACT FROM AUTHOR]