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Ginkgo biloba extract (EGb761) protects against aging-related caspase-mediated apoptosis in rat cochlea.
- Source :
- Acta Oto-Laryngologica; Oct2010, Vol. 130 Issue 10, p1101-1112, 12p, 1 Chart, 7 Graphs
- Publication Year :
- 2010
-
Abstract
- Conclusions: EGb761 treatment has a significant benefit with an early and preventive effect, reversing the deleterious effect of aging in the integrity of the rat cochlea, even in the late stage of the rat lifespan. Objectives: We previously reported a significant relationship between aging and apoptosis in the rat cochlea. This study was designed to investigate the effects of Ginkgo biloba leaf extract (EGb761) on age-associated cochlear caspase activation. Methods: Sprague-Dawley rats ( n = 80) divided into two groups according to their age (4 months old, younger, YR, and 12 months old, aged-mature, AM) were treated with 100 mg/kg/day body weight of EGb761 extract dissolved in tap water for two periods: 4 and 12 months. Then cochleae were harvested to measure caspase activities, ATP levels, total superoxide dismutase (SOD) activity, and caspase-3 gene expression. Auditory steady-state responses (ASSR) threshold shifts were also measured before sacrifice of the rats. Results: EGb761 treatment prevents significantly aging-related caspase-induced activities within the cochleae in YR and AM rats. In the short EGb761 treatment, YR rats showed lower levels of caspase-3/7 than AM rats. In contrast, longer treatment did not show significant differences between YR and AM rats. Reduced caspase-3/7 activity in presence of EGb761 correlates with significant improvements of ASSR threshold shifts. [ABSTRACT FROM AUTHOR]
- Subjects :
- GINKGO
ACADEMIC medical centers
ADENOSINE triphosphate
AGING
ANALYSIS of variance
ANIMAL experimentation
APOPTOSIS
BIOLOGICAL assay
COCHLEA
COLORIMETRY
COMPUTER software
DENSITOMETRY
FISHER exact test
GENE expression
HEARING levels
LUMINESCENCE spectroscopy
MITOCHONDRIA
POLYMERASE chain reaction
PRESBYCUSIS
PROTEINS
RATS
REGRESSION analysis
RESEARCH funding
SUPEROXIDE dismutase
WESTERN immunoblotting
DATA analysis
OXIDATIVE stress
TREATMENT duration
PHARMACODYNAMICS
PHYSIOLOGY
DRUG therapy
THERAPEUTICS
Subjects
Details
- Language :
- English
- ISSN :
- 00016489
- Volume :
- 130
- Issue :
- 10
- Database :
- Complementary Index
- Journal :
- Acta Oto-Laryngologica
- Publication Type :
- Academic Journal
- Accession number :
- 53561173
- Full Text :
- https://doi.org/10.3109/00016481003713657