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Remote pharmacological post-conditioning by intrathecal morphine: cardiac protection from spinal opioid receptor activation.
- Source :
- Acta Anaesthesiologica Scandinavica; Oct2010, Vol. 54 Issue 9, p1097-1104, 8p, 1 Diagram, 2 Charts, 3 Graphs
- Publication Year :
- 2010
-
Abstract
- Background: Intrathecal morphine pre-conditioning attenuates cardiac ischemia–reperfusion injury via activation of central opioid receptors. We hypothesized that intrathecal morphine also post-conditions the myocardium in the rat. Methods: Intrathecal morphine at 0.3 μg/kg (LMPC), 3 μg/kg (MMPC) or 30 μg/kg (HMPC) was administered for 5 min before 120-min reperfusion following 30-min ischemia. Infarct size as a percentage of area at risk (IS/AAR) was determined using triphenyltetrazolium staining. MMPC was repeated following the intrathecal administration of nor BNI, NTD, CTOP, or naloxone methiodide (NM), kappa, delta, mu and non-specific opioid receptor antagonists, respectively. The role of peripheral opioid, adenosine and calcitonin gene-related peptide (CGRP) receptors was examined by the intravenous administration of NM, 8-ρ-sulfophenyl theophylline (8-SPT) and human CGRP fragment (CGRP<subscript>8−37</subscript>), respectively. Results: Morphine post-conditioning at all three doses was cardioprotective (IS/AAR of LMPC=37±4%, MMPC=35±5%, HMPC=32±4%, control=50±5%, P<0.01). The prior administration of opioid receptor antagonists intrathecally, as well as intravenous 8-SPT and CGRP<subscript>8−37</subscript> receptor antagonists, abolished this effect (nor BNI+MMPC=47±7%, NTD+MMPC=49±7%, CTOP+MMPC=45±9%, NM+MMPC=47±6% 8-SPT+MPC=46±5% & CGRP<subscript>8−37</subscript>+MPC=53±6%, P=0.63). However, the intravenous administration of NM did not prevent the protective effect (34±4%, P<0.01). Conclusions: Intrathecal morphine administration can induce pharmacological cardiac post-conditioning as it involves opioid receptor centrally but non-opioid receptors peripherally. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00015172
- Volume :
- 54
- Issue :
- 9
- Database :
- Complementary Index
- Journal :
- Acta Anaesthesiologica Scandinavica
- Publication Type :
- Academic Journal
- Accession number :
- 53418495
- Full Text :
- https://doi.org/10.1111/j.1399-6576.2010.02295.x