LC-ED with an Acetylene Black–Dihexadecyl Hydrogen Phosphate Composite Film-Modified Electrode for in Vivo Analysis of Thiols in Rat Striatal Microdialysate.

Liquid chromatography with electrochemical detection (LC-ED), coupled with in vivo microdialysis sampling, has been used for analysis of thiols. An acetylene black–dihexadecyl hydrogen phosphate (AB–DHP) composite film-modified electrode was used as working electrode. The AB–DHP-modified electrode enabled efficient electrocatalytic oxidation of l-cysteine ( l-Cys) and glutathione (GSH) with relatively high sensitivity, stability, and longevity. The peak currents of l-Cys and GSH were linear in the concentrations ranges 2.0 × 10–2.0 × 10 and 3.0 × 10–5.0 × 10 mol L, respectively, with calculated detection limits (S/N = 3) of 1.0 × 10 and 2.0 × 10 mol L, respectively. The method has been successfully used to measure the amounts of l-Cys and GSH in striatal microdialysate of freely moving rats. [ABSTRACT FROM AUTHOR]