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Brain DNA damage and 70-kDa heat shock protein expression in CD1 mice exposed to extremely low frequency magnetic fields.

Authors :
Mariucci, Giuseppina
Villarini, Milena
Moretti, Massimo
Taha, Elena
Conte, Carmela
Minelli, Alba
Aristei, Cynthia
Ambrosini, Maria Vittoria
Source :
International Journal of Radiation Biology; Aug2010, Vol. 86 Issue 8, p701-710, 10p, 1 Chart, 4 Graphs
Publication Year :
2010

Abstract

Purpose: The question of whether exposure to extremely low frequency magnetic fields (ELF-MF), may contribute to cerebral cancer and neurodegeneration is of current interest. In this study we investigated whether exposure to ELF-MF (50 Hz-1 mT) harms cerebral DNA and induces expression of 70-kDa heat shock protein (hsp70). Materials and methods: CD1 mice were exposed to a MF (50 Hz-1 mT) for 1 or 7 days (15 h/day) and sacrificed either at the end of exposure or after 24 h. Unexposed and sham-exposed mice were used as controls. Mouse brains were dissected into cerebral cortex-striatum, hippocampus and cerebellum to evaluate primary DNA damage and hsp70 gene expression. Food intake, weight gain, and motor activity were also evaluated. Results: An increase in primary DNA damage was detected in all cerebral areas of the exposed mice sacrificed at the end of exposure, as compared to controls. DNA damage, as can be evaluated by the comet assay, appeared to be repaired in mice sacrificed 24 h after a 7-day exposure. Neither a short (15 h) nor long (7 days) MF-exposure induced hsp70 expression, metabolic and behavioural changes. Conclusions: These results indicate that in vivo ELF-MF induce reversible brain DNA damage while they do not elicit the stress response. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09553002
Volume :
86
Issue :
8
Database :
Complementary Index
Journal :
International Journal of Radiation Biology
Publication Type :
Academic Journal
Accession number :
52680708
Full Text :
https://doi.org/10.3109/09553001003789588