Acute neurosteroids inhibit the spinal reflex potentiation via GABAergic neurotransmission.

Recently, we demonstrated a chronic neurosteroid-dependent inhibition of activity-dependent spinal reflex potentiation (SRP), but it remains unclear whether neurosteroids acutely modulate SRP induction. This study shows progesterone as well as two of its 3α,5α-derivatives, allopregnalonone and 3α5α-tetrahydrodeoxycorticosterone (THDOC), to be capable of producing acute GABA_A receptor (GABA_AR)-dependent inhibition of SRP. When compared with test simulation (1 stimulation/30 s) of pelvic afferent nerves that evoked a baseline reflex activity in an external urethra sphincter electromyogram, repetitive stimulation (RS; 1 stimulation/1 s) induced SRP characterized by an increase in the evoked activity. Intrathecal progesterone (3-30 µM, 10 µl) at 10 rain before stimulation onset dose dependently prevented RS induction. Intrathecal allopregnalonone (10 µM, 10 µl it) and THDOC (10 µM, 10 µl it) also prevented the SRP caused by RS. Pretreatment with the GABA_AR antagonist bicuculline (10 µM, 10 µl it) at 1 min before progesterone/neurosteroid injection attenuated the inhibition of SRP caused by progesterone, allopregnanolone, and THDOC. Results suggest that progesterone and its neurosteroid metabolites may be crucial to the development of pelvic visceral neuropathic/postinflammatory pain and imply clinical use of neurosteroids, such as allopregnanolone and THDOC, for visceral pain treatment. [ABSTRACT FROM AUTHOR]