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The D4Z4 Macrosatellite Repeat Acts as a CTCF and AType Lamins-Dependent Insulator in Facio-Scapulo- Humeral Dystrophy.
- Source :
- PLoS Genetics; Feb2009, Vol. 5 Issue 2, p1-11, 11p, 1 Diagram, 5 Graphs
- Publication Year :
- 2009
-
Abstract
- Both genetic and epigenetic alterations contribute to Facio-Scapulo-Humeral Dystrophy (FSHD), which is linked to the shortening of the array of D4Z4 repeats at the 4q35 locus. The consequence of this rearrangement remains enigmatic, but deletion of this 3.3-kb macrosatellite element might affect the expression of the FSHD-associated gene(s) through position effect mechanisms. We investigated this hypothesis by creating a large collection of constructs carrying 1 to >11 D4Z4 repeats integrated into the human genome, either at random sites or proximal to a telomere, mimicking thereby the organization of the 4q35 locus. We show that D4Z4 acts as an insulator that interferes with enhancer-promoter communication and protects transgenes from position effect. This last property depends on both CTCF and A-type Lamins. We further demonstrate that both anti-silencing activity of D4Z4 and CTCF binding are lost upon multimerization of the repeat in cells from FSHD patients compared to control myoblasts from healthy individuals, suggesting that FSHD corresponds to a gain-of-function of CTCF at the residual D4Z4 repeats. We propose that contraction of the D4Z4 array contributes to FSHD physio-pathology by acting as a CTCF-dependent insulator in patients. [ABSTRACT FROM AUTHOR]
- Subjects :
- DYSTROPHY
HUMAN genome
MYOBLASTS
CELLS
PATIENTS
Subjects
Details
- Language :
- English
- ISSN :
- 15537390
- Volume :
- 5
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- PLoS Genetics
- Publication Type :
- Academic Journal
- Accession number :
- 51837956
- Full Text :
- https://doi.org/10.1371/journal.pgen.1000394