Inhibitory effects of eutigosides isolated from Eurya emarginata on the inflammatory mediators in RAW264.7 cells.

The anti-inflammatory activity of Eurya emarginata (Thumb) Makino, of which leaves have been traditionally used to treat ulcers or diuretic in Jeju Island, has been investigated in the present study. Through the phytochemical study from the methanol extract of E emaginiata, eutigosides and were isolated as the active components. Sseveral inflammatory markers including TNF-α, IL 1β, IL-6, NO, INOS, and COX-2 were examined. Eutigosides and potentially inhibited production of pro-inflammatory cytokines (IL-6 and TNF-α) in a dose-dependent manner. Additionally, the intracellular contents of INOS protein were markedly decreased after treatment with eutigosides and The inhibition of iNOS activity was correlated with the decrease in nitrite levels. These results suggest that eutigoside and from E. emarginata may have anti-inflammatory activity through the inhibition of pro-inflammatory cytokines (TNF-α and IL-6), INOS and COX-2. [ABSTRACT FROM AUTHOR]