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Micropropagation of Lepidium virginicum (Brassicaceae), a plant with antiprotozoal activity.

Authors :
Osuna, Lidia
Tapia-Pérez, María
Figueroa, Odette
Jiménez-Ferrer, Enrique
Garduño-Ramírez, María
González-Garza, María
Carranza-Rosales, Pilar
Cruz-Vega, Delia
Source :
In Vitro Cellular & Developmental Biology Plant; Nov2006, Vol. 42 Issue 6, p596-600, 5p
Publication Year :
2006

Abstract

Micropropagation is a technique to ensure a constant and uniform source of medicinal plants. In this report, we describe the micropropagation of Lepidium virginicum L. ( Brassicaceae), a wild plant used as an antiamoebic in traditional Mexican medicine. In vitro-germinated seeds were cultured in Murashige and Skoog (MS) medium to obtain pathogen-free cotyledons, hypocotyls, and apical bud (AB) explants. For induction of morphogenesis, the effect of cytokinins, benzyladenine (BA) and kinetin (KN), combined with auxin, indole-3-acetic acid (IAA) was evaluated. The best rate of shoot proliferation was induced 15 d after culture on MS mineral medium supplemented with IAA∶KN (0.57∶13.94 μ M) from AB explants. Maximum shoot elongation was achieved without plant growth regulators. The effect of indole-3-butyric acid (IBA) (14.76 μ M) was evaluated for in vitro root induction; 60 d after culture all the shoots had developed roots. All rooted plants were successfully transferred to pots and 100% acclimatized in ex vitro conditions. The methanol extracts from the micropropagated active explants of L. virginicum showed and IC<subscript>50</subscript> antiprotozoal value between 141.90 and 268.53 μg ml<superscript>−1</superscript>. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
10545476
Volume :
42
Issue :
6
Database :
Complementary Index
Journal :
In Vitro Cellular & Developmental Biology Plant
Publication Type :
Academic Journal
Accession number :
51561090
Full Text :
https://doi.org/10.1079/IVP2006826