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Production of d-arabitol by a newly isolated Kodamaea ohmeri.

Authors :
Hong-Yang Zhu
Hong Xu
Xiao-Yan Dai
Yang Zhang
Han-Jie Ying
Ping-Kai Ouyang
Source :
Bioprocess & Biosystems Engineering; Jun2010, Vol. 33 Issue 5, p565-571, 7p, 4 Charts, 2 Graphs
Publication Year :
2010

Abstract

A new yeast, isolated from natural osmophilic sources, produces d-arabitol as the main metabolic product from glucose. According to 18S rRNA analysis, the NH-9 strain belongs to the genus Kodamaea. The optimal culture conditions for inducing production of d-arabitol were 37 °C, neutral pH, 220 rpm shaking, and 5% inoculum. The yeast produced 81.2 ± 0.67 g L<superscript>−1</superscript> d-arabitol from 200 g L<superscript>−1</superscript> d-glucose in 72 h with a yield of 0.406 g g<superscript>−1</superscript> glucose and volumetric productivity $$ Q_{\text{P}} $$ of 1.128 g L<superscript>−1</superscript> h<superscript>−1</superscript>. Semi-continuous repeated-batch fermentation was performed in shaker-flasks to enhance the process of d-arabitol production by Kodamaea ohmeri NH-9 from d-glucose. Under repeated-batch culture conditions, the highest volumetric productivity was 1.380 g L<superscript>−1</superscript> h<superscript>−1</superscript>. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
16157591
Volume :
33
Issue :
5
Database :
Complementary Index
Journal :
Bioprocess & Biosystems Engineering
Publication Type :
Academic Journal
Accession number :
50444489
Full Text :
https://doi.org/10.1007/s00449-009-0378-x