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Renilla luciferase as a vital reporter for chloroplast gene expression in Chlamydomonas.

Renilla luciferase as a vital reporter for chloroplast gene expression in Chlamydomonas.

Authors :
Minko, I.
Holloway, S. P.
Nikaido, S.
Carter, M.
Odom, O. W.
Johnson, C. H.
Herrin, D. L.
Source :
Molecular & General Genetics MGG; Nov1999, Vol. 262 Issue 3, p421-425, 5p
Publication Year :
1999

Abstract

The use of luciferases as reporters of gene expression in living cells has been extended to the chloroplast genome. We show that the luciferase from the soft coral Renilla reniformis (Rluc) can be successfully expressed in the chloroplast of Chlamydomonas reinhardtii. Expression of the rluc cDNA was driven by the promoter and 5′ untranslated regions of the atpA gene. Western analysis with an anti-Rluc antibody detected a single polypeptide of 38 kDa in the luminescent cells. This is 3 kDa larger than native Rluc, and suggests that translation of the chimeric mRNA begins at the atpA start codon, 29 codons upstream from the rluc start site. We also show that the luminescence of the transformants was sufficient to enable imaging of colonies using a cooled CCD camera. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00268925
Volume :
262
Issue :
3
Database :
Complementary Index
Journal :
Molecular & General Genetics MGG
Publication Type :
Academic Journal
Accession number :
50143344
Full Text :
https://doi.org/10.1007/s004380051101