Photochemical Properties of Photosystem 2 in Primary Leaves of Barley Seedlings Grown Under Various Blue or Red Irradiances.

Photosystem 2 (PS2)-driven electron transfer was studied in primary leaves of barley ( Hordeum vulgare L.) seedlings grown under various photon fluxes (0.3–170.0 μmol m⁻² s⁻¹) of blue (BR) or red (RR) radiation using modulated chlorophyll fluorescence. The F_v/F_m ratio was 0.78–0.79 in leaves of all radiation variants, except in seedlings grown under BR or RR of 0.3 μmol m⁻² s⁻¹. The extent of the photochemical phase of the polyphasic F_v rise induced by very strong “white light” was similar in leaves of all radiation treatments. Neither radiation quality nor photon flux under plant cultivation influenced the amount of non Q_B-transferring centres of PS2 except in leaves of seedlings grown under BR of 0.3 μmol m⁻² s⁻¹, in which the amount of such centres increased threefold. Both BR and RR stimulated the development of photochemically competent PS2 at photon fluxes as low as 3 μmol m⁻² s⁻¹. Three exponential components with highly different half times were distinguished in the kinetics of F_v dark decay. This indicates different pathways of electron transfer from Q_A ⁻, the reduced primary acceptor of PS2, to other acceptors. Relative magnitudes of the individual decay components did not depend on the radiation quality or the photon flux during plant cultivation. Significant differences were found, however, between plants grown under BR or RR in the rate of the middle and fast components of F_v dark decay, which showed 1.5-times faster intersystem linear electron transport in BR-grown leaves. [ABSTRACT FROM AUTHOR]