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Evaluation of endothelial cell integrins after in vitro contact with polyethylene terephthalate.

Evaluation of endothelial cell integrins after in vitro contact with polyethylene terephthalate.

Authors :
Cenni, E.
Granchi, D.
Verri, E.
Remiddi, G.
Cavedagna, D.
Di Leo, A.
Source :
Journal of Materials Science: Materials in Medicine; Apr2001, Vol. 12 Issue 4, p345-349, 5p
Publication Year :
2001

Abstract

The aim of this research was to evaluate the effect of polyethylene terephthalate (Woven Dacron) on the expression of endothelial integrins. Human umbilical vein endothelial cells were cultured on the material for 24 h. The integrins VLA-2 (α<subscript>2</subscript>β<subscript>1</subscript>-CD49b/CD29), receptor for laminin and collagen, VLA-5 (α<subscript>5</subscript>β<subscript>1</subscript>-CD49e/CD29), receptor for fibronectin, VLA-6 (α<subscript>6</subscript>β<subscript>1</subscript>-CD49f/CD29), receptor for laminin, and αVβ<subscript>3</subscript>-CD51/CD61 (receptor for vitronectin) were evaluated by flow cytometry. After contact with polyethylene terephthalate, a slight but significant decrease in the percentage of both CD29 and CD49e positive cells was observed, which suggests a lower number of cells expressing the fibronectin receptor α<subscript>5</subscript>β<subscript>1</subscript>. Moreover, a significant increase in the mean channel for CD49b and for the vitronectin receptor CD51/CD61 was observed. The reduction in the fibronectin receptor could account for the poor endothelialization observed in vivo on polyethylene terephthalate. The increased expression of the vitronectin receptor, favoring the migration of smooth muscle cells, could give some information about the pathogenesis of intimal hyperplasia, which is a complication of vascular grafts. © 2001 Kluwer Academic Publishers [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09574530
Volume :
12
Issue :
4
Database :
Complementary Index
Journal :
Journal of Materials Science: Materials in Medicine
Publication Type :
Academic Journal
Accession number :
50045732
Full Text :
https://doi.org/10.1023/A:1011203322367