Back to Search Start Over

Protein Patterns, Characterized by Computer Image Analysis, of Lentil Embryo Axes Germinating Under Salt Stress.

Authors :
Dell'Aquila, A.
Source :
Biologia Plantarum; Jun2004, Vol. 48 Issue 2, p237-242, 6p
Publication Year :
2004

Abstract

Following 16, 40 and 64 h exposure to 0.33 M NaCl given after 8 h water imbibition, lentil seeds showed a gradual decrease of germination upon their transfer to water. These salt related changes were accompanied by modifications in the protein patterns of embryo axes as revealed by two-dimensional electrophoresis separation and by the computer image analysis of protein spots. In comparison with 8 h water imbibed seeds, prominent proteins comprised between the 5.1 – 7.6 pH isoelectric point in the first dimension and 75 – 50 kDa molecular mass in the second dimension showed a significant increase in their abundance as salt exposure increased. On transfer to water to complete germination, the content of many of these proteins decreased at 24h in 2 – 3 cm length embryo axes in comparison with the corresponding embryo axes of seeds continuously imbibed in water for 24 h. Some groups of proteins ranging between 15.5 – 17.3 kDa, already present after 8 h water imbibition, were not detectable after 24 h but were expressed in seeds exposed to NaCl and transferred to water for 24 h. Up- and down-regulated proteins in lentil embryo axes, imbibed under non-lethal salt stress conditions, have been tentatively identified by comparison with the protein map of germinating seeds of the model plant Arabidopsis. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00063134
Volume :
48
Issue :
2
Database :
Complementary Index
Journal :
Biologia Plantarum
Publication Type :
Academic Journal
Accession number :
50016075
Full Text :
https://doi.org/10.1023/B:BIOP.0000033450.16985.07