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Protein Patterns, Characterized by Computer Image Analysis, of Lentil Embryo Axes Germinating Under Salt Stress.
- Source :
- Biologia Plantarum; Jun2004, Vol. 48 Issue 2, p237-242, 6p
- Publication Year :
- 2004
-
Abstract
- Following 16, 40 and 64 h exposure to 0.33 M NaCl given after 8 h water imbibition, lentil seeds showed a gradual decrease of germination upon their transfer to water. These salt related changes were accompanied by modifications in the protein patterns of embryo axes as revealed by two-dimensional electrophoresis separation and by the computer image analysis of protein spots. In comparison with 8 h water imbibed seeds, prominent proteins comprised between the 5.1 – 7.6 pH isoelectric point in the first dimension and 75 – 50 kDa molecular mass in the second dimension showed a significant increase in their abundance as salt exposure increased. On transfer to water to complete germination, the content of many of these proteins decreased at 24h in 2 – 3 cm length embryo axes in comparison with the corresponding embryo axes of seeds continuously imbibed in water for 24 h. Some groups of proteins ranging between 15.5 – 17.3 kDa, already present after 8 h water imbibition, were not detectable after 24 h but were expressed in seeds exposed to NaCl and transferred to water for 24 h. Up- and down-regulated proteins in lentil embryo axes, imbibed under non-lethal salt stress conditions, have been tentatively identified by comparison with the protein map of germinating seeds of the model plant Arabidopsis. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00063134
- Volume :
- 48
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Biologia Plantarum
- Publication Type :
- Academic Journal
- Accession number :
- 50016075
- Full Text :
- https://doi.org/10.1023/B:BIOP.0000033450.16985.07