The Genetic Organization of a 2,966 basepair DNA Fragment of a Single Capsid Nucleopolyhedrovirus Isolated from Trichoplusia ni.

In order to investigate the genomic organization of the Trichoplusia ni Single Capsid Nucleopolyhedrovirus (TnSNPV), a 2,966 basepairs (bp) genomic fragment was sequenced. The fragment was found to contain five open reading frames (ORFs) homologous to baculovirus genes, including p26, fibrillin ( p10), AcMNPV ORF-29, late expression factor 6 ( lef-6) and the C-terminal portion of p 74, on either strand of DNA. Predicted amino acid sequences for the ORFs were compared and identity values of between 12% and 54% were observed. TnSNPV has previously been tentatively identified as a member of the Group II NPVs. Clustering and arrangement of the TnSNPV genes were similar to the clustering reported for SeMNPV, confirming TnSNPV as a Group II NPV. [ABSTRACT FROM AUTHOR]