Back to Search
Start Over
Down-regulation of liver RNA breakdown by turpentine administration in the starved rat: Autophagy and relevant factors.
- Source :
- Inflammation Research; Apr1999, Vol. 48 Issue 4, p210-217, 8p
- Publication Year :
- 1999
-
Abstract
- Objective and design: To determine whether the inhibition of RNA breakdown observed in ad libitum fed rats 24 h after turpentine administration [1] still occurs in inflamed rats fasted for 24 h and to examine the mechanism and factors involved.¶ Methods: RNA breakdown was measured during cyclic in situ perfusion of livers by the accumulation of [<superscript>14</superscript>C] cytidine after in vivo RNA labelling. Autophagic activity was determined by the morphometric analysis of lysosomal structures.¶ Results: The decrease in RNA breakdown (53%) observed in the inflamed rats was accompanied by a 38% drop in the fractional cytoplasmic volume of initial and digestive autophagic vacuoles. Among amino acids, only the portal levels of glutamate were significantly enhanced by 83%. In vivo suppression of glucocorticoid activity using RU 38486 in inflamed rats did not affect the inhibition of RNA breakdown.¶ Conclusions: The results show that turpentine-induced inflammation in fasted rats inhibits RNA degradation as well as autophagy and that glucocorticoids do not seem to be involved. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10233830
- Volume :
- 48
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Inflammation Research
- Publication Type :
- Academic Journal
- Accession number :
- 49906976
- Full Text :
- https://doi.org/10.1007/s000110050448