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Quantitative Determination of 14 Major Constituents in the Herbal Preparation Luan-Pao-Prescription Using HPLC Coupled with Photodiode Array Detection.
- Source :
- Chromatographia; Aug2007, Vol. 66 Issue 3/4, p267-270, 4p
- Publication Year :
- 2007
-
Abstract
- High performance liquid chromatography coupled with photodiode array detection (HPLC–DAD) was employed for the determination of 14 major constituents in traditional Chinese herbal formula ‘Luan-Pao-Prescription’. Two different chromatographic determinations for the 14 constituents were carried out to control the quality of this herbal formula. The sample was extracted under ultrasonic extraction with 75% methanol at the frequency of 42 kHz for 45 min at room temperature. The chromatographic separation was performed on a Zorbax SB-C<subscript>18</subscript>. The mobile phase was linear gradient elution of water with 0.5% ( v/v) acetic acid (A) and acetonitrile (B) column with linear gradient elution procedure. The flow rate was set at 1.0 mL min<superscript>−1</superscript> and the column temperature was kept at 30 °C. The calibration curve for the determination of all the analytes showed good linearity over the investigated ranges ( R <superscript>2</superscript> > 0.999). The intra- and inter-day assay precision (RSD) of 14 analytes were in the range of 0.2–3.9% and the recoveries were between 92.4 and 104.4%. The validated method was successfully applied to the determination of 14 major constituents in LPP. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00095893
- Volume :
- 66
- Issue :
- 3/4
- Database :
- Complementary Index
- Journal :
- Chromatographia
- Publication Type :
- Academic Journal
- Accession number :
- 49523368
- Full Text :
- https://doi.org/10.1365/s10337-007-0296-5