Extraction and liquid-chromatographic determination of amphotericin B in oil-water lecithin-based microemulsions.

A method employing extraction then reproducible reversed-phase high-performance liquid chromatography (RPHPLC) with monitoring of the drug by absorbance at 405 nm has been developed and validated for the determination of amphotericin B in oil-water lecithin-based microemulsions. The precision and accuracy of the method are excellent ( SD 2.4% and 4.2%, respectively). The established linearity range was 10–60 μg mL⁻¹ ( r ²=0.9967). The recovery of amphotericin B from spiked placebo was >90% over the linear range. The extraction procedure was simple and the HPLC conditions were able to separate the drug from its degradation products and excipients. The method has been used successfully for determining the amphotericin B content of microemulsions and for evaluating the chemical stability of amphotericin B-loaded microemulsions. [ABSTRACT FROM AUTHOR]