The effect of BAP on the gene expression of a small GTP-binding protein, Rho1Ps in a shoot apex of garden pea.

The effect of a synthetic cytokinin, 6-benzylaminopurine (BAP), on gene expression of Rho1Ps in shoot apex segments of garden pea was examined by Northern blot hybridization using³²P-labelled 560 bp partial Rho1Ps cDNA fragment as the probe. The application of 10¹² M BAP, the lowest concentration tested, for 24h to the segments strongly activated the synthesis of Rho1Ps transcripts as much as 7 times compared with those in BAP-untreated segments. In addition, the Rho1Ps transcripts synthesis was enhanced by BAP in a concentration dependent manner, suggesting that BAP is concretly involved in the Rho1Ps gene expression in the segments. A time course analysis of BAP on Rho1Ps gene expression exhibited that BAP was closely related not only to the Rho1Ps transcripts synthesis but also to the degradation of Rho1Ps transcripts. [ABSTRACT FROM AUTHOR]