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Improvement in organophosphorus hydrolase activity of cell surface-engineered yeast strain using Flo1p anchor system.
- Source :
- Biotechnology Letters; May2010, Vol. 32 Issue 5, p655-659, 5p, 2 Diagrams, 1 Graph
- Publication Year :
- 2010
-
Abstract
- Organophosphorus hydrolase (OPH) hydrolyzes organophosphorus esters. We constructed the yeast-displayed OPH using Flo1p anchor system. In this system, the N-terminal region of the protein was fused to Flo1p and the fusion protein was displayed on the cell surface. Hydrolytic reactions with paraoxon were carried out during 24 h of incubation of OPH-displaying cells at 30°C. p-Nitrophenol produced in the reaction mixture was detected by HPLC. The strain with highest activity showed 8-fold greater OPH activity compared with cells engineered using glycosylphosphatidylinositol anchor system, and showed 20-fold greater activity than Escherichia coli using the ice nucleation protein anchor system. These results indicate that Flo1p anchor system is suitable for display of OPH in the cell surface-expression systems. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01415492
- Volume :
- 32
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Biotechnology Letters
- Publication Type :
- Academic Journal
- Accession number :
- 49087358
- Full Text :
- https://doi.org/10.1007/s10529-010-0204-1