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Conditioned medium from neonatal rat olfactory ensheathing cells promotes the survival and proliferation of spiral ganglion cells.
- Source :
- Acta Oto-Laryngologica; Mar2010, Vol. 130 Issue 3, p351-357, 7p, 1 Diagram, 1 Graph
- Publication Year :
- 2010
-
Abstract
- Conclusion: Neonatal olfactory ensheathing cells conditioned medium (OEC-CM) can promote growth and proliferation of neonatal spiral ganglion cells (SGCs), as well as protecting them from apoptosis. Objective: To investigate the effects of neonatal OEC-CM on the survival, apoptosis, and proliferation of spiral ganglion. Methods: SGCs were cultured with OEC-CM, the conditioned medium from olfactory nerve fibroblasts (ONF-CM) or control medium containing 10% serum (DF12-10S). After different periods of culture, the cell types present were identified by immunofluorescent confocal imaging analysis, cell proliferation was determined by BrdU incorporation, and apoptosis was detected with the double-labeled NeuroTACS in situ Apoptosis Detection Kit. Results: SGCs cultured with regular control medium died off via apoptosis. Culturing cells with ONF-CM had no significant effects on the natural process of survival and apoptosis of SGCs. However, culture with OEC-CM significantly increased survival and decreased the apoptotic percentage of SGCs. In addition, OEC-CM temporarily stimulated the proliferative capacity of SGCs on the third day of culture. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00016489
- Volume :
- 130
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Acta Oto-Laryngologica
- Publication Type :
- Academic Journal
- Accession number :
- 48061152
- Full Text :
- https://doi.org/10.3109/00016480903154256