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Biotransformation of the antitumor intermediate 5-fluorouridine by recombinant Escherichia coli with high pyrimidine nucleoside phosporylase activity.
- Source :
- Biocatalysis & Biotransformation; Mar2010, Vol. 28 Issue 2, p130-136, 7p, 1 Diagram, 1 Chart, 5 Graphs
- Publication Year :
- 2010
-
Abstract
- 5-Fluorouridine (5-FUrd) is a precursor of the widely used antitumor drug doxifluridine. We have produced 5-FUrd by biotransformation by cloning the gene encoding pyrimidine nucleoside phosphorylase (PyNPase) from Enterobacter aero-genes CMCC (B) 45103 and expression in Escherichia coli BL21 (DE3), resulting in recombinant E. coli BL21 (DE3)/ pET28a-PyNPase. After medium optimization, the PyNPase activity in the fermentation broth was 1613 U mg<superscript>–1</superscript>, which was 54-fold that of E. aerogenes. Under optimal conditions (cell concentration, 0.5 g L<superscript>–1</superscript>; uridine, 10 mM; 5-fluorouracil, 45 mM; temperature, 50°C; pH, 7.8), more than 90% of uridine was converted to 5-FUrd, suggesting that this is a valuable tool for application in the preparation of antiviral and antitumor drugs. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10242422
- Volume :
- 28
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Biocatalysis & Biotransformation
- Publication Type :
- Academic Journal
- Accession number :
- 48026967
- Full Text :
- https://doi.org/10.3109/10242420903505842