Back to Search
Start Over
NF-κB activation stimulates osteogenic differentiation of mesenchymal stem cells derived from human adipose tissue by increasing TAZ expression.
- Source :
- Journal of Cellular Physiology; Apr2010, Vol. 223 Issue 1, p168-177, 10p, 1 Diagram, 1 Chart, 6 Graphs
- Publication Year :
- 2010
-
Abstract
- Tumor necrosis factor-alpha (TNF-α) is a skeletal catabolic agent that stimulates osteoclastogenesis and inhibits osteoblast function. Although TNF-α inhibits the mineralization of osteoblasts, the effect of TNF-α on mesenchymal stem cells (MSC) is not clear. In this study, we determined the effect of TNF-α on osteogenic differentiation of stromal cells derived from human adipose tissue (hADSC) and the role of NF-κB activation on TNF-α activity. TNF-α treatment dose-dependently increased osteogenic differentiation over the first 3 days of treatment. TNF-α activated ERK and increased NF-κB promoter activity. PDTC, an NF-κB inhibitor, blocked the osteogenic differentiation induced by TNF-α and TLR-ligands, but U102, an ERK inhibitor, did not. Overexpression of miR-146a induced the inhibition of IRAK1 expression and inhibited basal and TNF-α- and TLR ligand-induced osteogenic differentiation. TNF-α and TLR ligands increased the expression of transcriptional coactivator with PDZ-binding motif (TAZ), which was inhibited by the addition of PDTC. A ChIP assay showed that p65 was bound to the TAZ promoter. TNF-α also increased osteogenic differentiation of human gastroepiploic artery smooth muscle cells. Our data indicate that TNF-α enhances osteogenic differentiation of hADSC via the activation of NF-κB and a subsequent increase of TAZ expression. J. Cell. Physiol. 223: 168–177, 2010. © 2009 Wiley-Liss, Inc. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00219541
- Volume :
- 223
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Journal of Cellular Physiology
- Publication Type :
- Academic Journal
- Accession number :
- 47805755
- Full Text :
- https://doi.org/10.1002/jcp.22024