Back to Search Start Over

Identification of a Novel Major Quantitative Trait Locus Controlling Distribution of Cd Between Roots and Shoots in Rice.

Authors :
Ueno, Daisei
Koyama, Emi
Kono, Izumi
Ando, Tsuyu
Yano, Masahiro
Ma, Jian Feng
Source :
Plant & Cell Physiology; Dec2009, Vol. 50 Issue 12, p2223-2233, 11p, 7 Graphs
Publication Year :
2009

Abstract

Accumulation of Cd in rice grain is a serious concern of food safety since rice as a staple food is a major source of Cd intake in Asian countries. However, the mechanisms controlling Cd accumulation in rice are still poorly understood. Herein, we report both physiological and genetic analysis of two rice cultivars contrasting in Cd accumulation, which were screened from a core collection of rice cultivars. The cultivar Anjana Dhan (Indica) accumulated much higher levels of Cd than Nipponbare (Japonica) in the shoots and grains when grown in both soil and solution culture. A short-term uptake experiment (20 min) showed that Cd uptake by Nipponbare was higher than that by Anjana Dhan. However, the concentration of Cd in the shoot and xylem sap was much higher in Anjana Dhan than in Nipponbare. Of the Cd taken up by the roots, <4% was translocated to the shoots in Nipponbare, compared with 10–25% in Anjana Dhan, indicating a higher root-to-shoot translocation of Cd in the latter. A quantitative trait locus (QTL) analysis for Cd accumulation was performed using an F2 population derived from Anjana Dhan and Nipponbare. A QTL with large effect for Cd accumulation was detected on the short arm of chromosome 7, explaining 85.6% of the phenotypic variance in the shoot Cd concentration of the F2 population. High accumulation is likely to be controlled by a single recessive gene. A candidate genomic region was defined to <1.9 Mb by means of substitution mapping. [ABSTRACT FROM PUBLISHER]

Details

Language :
English
ISSN :
00320781
Volume :
50
Issue :
12
Database :
Complementary Index
Journal :
Plant & Cell Physiology
Publication Type :
Academic Journal
Accession number :
47144861
Full Text :
https://doi.org/10.1093/pcp/pcp160