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Bioengineered Trachea With Fibroblasts in a Rabbit Model.
- Source :
- Annals of Otology, Rhinology & Laryngology; Nov2009, Vol. 118 Issue 11, p796-804, 9p
- Publication Year :
- 2009
-
Abstract
- Objectives: Although our group has had mostly successful results with clinical application of a tracheal prosthesis, delayed epithelial regeneration remains a problem. In our previous studies using rats, it was demonstrated that tracheal fibroblasts accelerated proliferation and differentiation of the tracheal epithelium in vitro and in vivo. The purpose of this study was to evaluate the effects of fibroblasts on epithelial regeneration in larger tracheal defects in rabbits. Methods: We developed a bioengineered scaffold, the luminal surface of which was coated with fibroblasts. This scaffold was implanted into tracheal defects in 12 rabbits (bioengineered group), and scaffolds without fibroblasts were implanted in 12 rabbits (control group). The regenerated epithelium was histologically examined by light microscopy, scanning electron microscopy, and immunohistochemical studies. Results: In the bioengineered group, a stratified squamous epithelium was observed on the surface 7 days after transplantation. However, in the control group, the scaffolds were exposed. Fourteen days after implantation, a columnar ciliated epithelium was observed in the bioengineered group. The average thickness of the regenerated epithelium in the bioengineered group was significantly greater than that in the control group. Conclusions: This study indicated that fibroblasts had a stimulatory effect that hastened regeneration of the epithelium in large tracheal defects. [ABSTRACT FROM AUTHOR]
- Subjects :
- PROSTHETICS
EPITHELIAL cells
FIBROBLASTS
REGENERATION (Biology)
ELECTRON microscopy
Subjects
Details
- Language :
- English
- ISSN :
- 00034894
- Volume :
- 118
- Issue :
- 11
- Database :
- Complementary Index
- Journal :
- Annals of Otology, Rhinology & Laryngology
- Publication Type :
- Academic Journal
- Accession number :
- 45481617
- Full Text :
- https://doi.org/10.1177/000348940911801109