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Dendritic cell activating peptides induce distinct cytokine profiles.
- Source :
- International Immunology; Nov2006, Vol. 18 Issue 11, p1563-1573, 11p
- Publication Year :
- 2006
-
Abstract
- High-mobility group box 1 protein (HMGB1), a DNA-binding nuclear and cytosolic protein, is a pro-inflammatory cytokine released by monocytes and macrophages. HMGB1 as well as its B box domain induce maturation of human dendritic cells (DCs). This report demonstrates that the B box domain induces phenotypic maturation of murine bone marrow-derived dendritic cells (BM-DCs) as evidenced by increased CD86, CD40 and MHC-II expression. The B box domain enhanced secretion of pro-inflammatory cytokines and chemokines: IL-1β, IL-2, IL-5, IL-8, IL-12 and tumor necrosis factor (TNF)-α, but not IL-6 and IL-10. Furthermore, four peptides whose sequences correspond to different regions of HMGB1 induced production of IL-1β, IL-2 and IL-12 (p70), but not IL-10 and IL-6 in mouse BM-DCs. Interestingly, these peptides differed in their capacity to induce TNF-α, IL-5, IL-18 and IL-8. B box domain as well as peptide-activated DCs acted as potent stimulators of allogeneic T cells in a mixed leukocyte reaction. DCs exposed to HMGB1 peptides induced proliferation of ovalbumin-specific syngeneic T cells. These DC-activating peptides could serve as an adjuvant in immunotherapeutic or vaccine context and the selective activity of these different peptides suggests a means to customize the functional properties of DCs. [ABSTRACT FROM PUBLISHER]
Details
- Language :
- English
- ISSN :
- 09538178
- Volume :
- 18
- Issue :
- 11
- Database :
- Complementary Index
- Journal :
- International Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 44424195
- Full Text :
- https://doi.org/10.1093/intimm/dxl089