Automated protein (re)sequencing with MS/MS and a homologous database yields almost full coverage and accuracy.

Motivation: The bottom-up tandem mass spectrometry (MS/MS) is regularly used in proteomics nowadays for identifying proteins from a sequence database. De novo sequencing software is also available for sequencing novel peptides with relatively short sequence lengths. However, automated sequencing of novel proteins from MS/MS remains a challenging problem. [ABSTRACT FROM PUBLISHER]