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Active Mask Segmentation of Fluorescence Microscope Images.
- Source :
- IEEE Transactions on Image Processing; Aug2009, Vol. 18 Issue 8, p1817-1829, 13p, 11 Diagrams, 2 Charts
- Publication Year :
- 2009
-
Abstract
- We propose a new active mask algorithm for the segmentation of fluorescence microscope images of punctate patterns. It combines the (a) flexibility offered by active-contour methods, (b) speed offered by muitiresolution methods, (c) smoothing offered by multiscale methods, and (d) statistical modeling offered by region-growing methods into a fast and accurate segmentation tool. The framework moves from the idea of the "contour" to that of "inside and outside" or masks, allowing for easy multidimensional segmentation. It adapts to the topology of the image through the use of multiple masks. The algorithm is almost invariant under initialization, allowing for random initialization, and uses a few easily tunable parameters. Experiments show that the active mask algorithm matches the ground truth well and outperforms the algorithm widely used in fluorescence microscopy, seeded watershed, both qualitatively, as well as quantitatively. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10577149
- Volume :
- 18
- Issue :
- 8
- Database :
- Complementary Index
- Journal :
- IEEE Transactions on Image Processing
- Publication Type :
- Academic Journal
- Accession number :
- 43297476
- Full Text :
- https://doi.org/10.1109/TIP.2009.2021081