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Evaluation of the Contribution of Major T Cell Subsets to IFN-γ Production in TB Infection by ELISPOT.
- Source :
- Immunological Investigations; Jul2009, Vol. 38 Issue 5, p341-349, 9p, 1 Graph
- Publication Year :
- 2009
-
Abstract
- Interferon gamma remains a key effector molecule that is still widely used as the most informative biomarker for screening human immune responses against tuberculosis, particularly in ELISPOT assays. We investigated the participation of CD4+ and CD8+ T lymphocytes in the PBMC responses to Mycobacterium tuberculosis (Mtb) specific antigens in 33 TB cases and 49 contacts. Responses to ESAT-6 were higher than CFP-10. There was no significant difference in responses to both Mtb antigens between cases and contacts. PBMCs response to ESAT-6 but not CFP-10 in cases was significantly reduced by depletion of CD4+ cells whereas CD8+ cell depletion had no impact. In conclusion, ESAT-6 is a more recognized antigen in this population, and CD4+ lymphocytes are the main participants in IFN-γ response by ELISPOT. Thus, a decline of CD4+ T lymphocytes below a critical level might affect the sensitivity of IFN-γ release assays for detecting Mtb infection. [ABSTRACT FROM AUTHOR]
- Subjects :
- T cells
TUBERCULOSIS
IMMUNE response
BIOMARKERS
ANTIGENS
INFECTION
Subjects
Details
- Language :
- English
- ISSN :
- 08820139
- Volume :
- 38
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Immunological Investigations
- Publication Type :
- Academic Journal
- Accession number :
- 42746405
- Full Text :
- https://doi.org/10.1080/08820130902748744