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AT2 Receptor Deficiency Attenuates Adipocyte Differentiation and Decreases Adipocyte Number in Atherosclerotic Mice.
- Source :
- American Journal of Hypertension; Jul2009, Vol. 22 Issue 7, p784-791, 8p, 1 Chart, 7 Graphs
- Publication Year :
- 2009
-
Abstract
- BackgroundPrevious reports indicated that blockade of AT<subscript>1</subscript> receptor stimulation attenuated adipocyte dysfunction. However, the effects of AT<subscript>2</subscript> receptor stimulation on adipose tissue were not yet clear. In the present study, we examined the adipose tissue dysfunction in atherosclerotic apolipoprotein E knockout (ApoEKO) mice with AT<subscript>2</subscript> receptor deficiency.MethodsMale ApoEKO and AT<subscript>2</subscript> receptor/ApoE knockout (AT<subscript>2</subscript>/ApoEKO) mice at 6 weeks of age were treated with a normal diet or a high-cholesterol diet (HCD: 1.25% cholesterol). Markers for adipocyte differentiation and inflammation in adipose tissue were assayed with real-time reverse-transcription-PCR and western blot.ResultsCompared with ApoEKO mice, AT<subscript>2</subscript>/ApoEKO mice with a normal diet showed only a decrease in expression of adiponectin and CCAAT/enhancer binding protein δ (C/EBPδ) in epididymal adipose tissue without changes in body weight, adipose tissue weight, and adipocyte number even at 6 months of age. After HCD for 4 weeks, the weight of both epididymal and retroperitoneal adipose tissue in AT<subscript>2</subscript>/ApoEKO mice was greater than that in ApoEKO mice without a change in body weight. Plasma concentrations of cholesterol and fatty acids were higher in AT<subscript>2</subscript>/ApoEKO mice than in ApoEKO mice. In adipose tissue of AT<subscript>2</subscript>/ApoEKO mice, the adipocyte number was decreased and the expression of peroxisome proliferator–activated receptor γ (PPARγ), C/EBPα, and aP2 was lower than that in ApoEKO mice, in association with an increase in nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity.ConclusionsThese results suggest that AT<subscript>2</subscript> receptor stimulation in adipose tissue is involved in the improvement of adipocyte differentiation and adipose tissue dysfunction in atherosclerotic model.American Journal of Hypertension 2009; doi:10.1038/ajh.2009.85American Journal of Hypertension 2009; 22, 7, 784–791. doi:10.1038/ajh.2009.85 [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 08957061
- Volume :
- 22
- Issue :
- 7
- Database :
- Complementary Index
- Journal :
- American Journal of Hypertension
- Publication Type :
- Academic Journal
- Accession number :
- 42223490
- Full Text :
- https://doi.org/10.1038/ajh.2009.85