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An in-vitro model for comparing the efficiency of wound-rinsing solutions.
- Source :
- Journal of Wound Care; Jun2009, Vol. 18 Issue 6, p229-236, 5p, 2 Color Photographs, 1 Diagram, 1 Graph
- Publication Year :
- 2009
-
Abstract
- Objective: To develop an in-vitro model to evaluate the efficiency of wound-rinsing solutions in removing adherent, hydrophobic, denatured proteins. We hypothesised that saline solutions would be less effective than surfactant-containing solutions in removing denatured proteins. Method: Prepared slides containing dried blood plasma or fibrin were incubated for up to one hour in histological troughs filled with one of four test solutions: physiological saline solution, Ringer's solution, a surfactant-containing solution and an antiseptic. The concentration of dissolved proteins was measured using a modified Biuret test. Results were analysed by plotting protein concentration against the incubation time. Results: During the incubation period, the protein concentration increased in all of the test solutions, with the lowest concentration reported in the two saline-based solutions. These stayed clear, while the surfactant-containing solution become opaque, indicating that the surfactant had encased hydrophic substances, such as denatured proteins. Conclusion: Ringer's solution and saline are inappropriate solvents for adhering wound coatings. A sterile, surfactant-containing wound rinsing solution contains the essential properties for thorough and gentle cleansing of chronic wounds. Declaration of Interest: None. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09690700
- Volume :
- 18
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Journal of Wound Care
- Publication Type :
- Academic Journal
- Accession number :
- 41789037
- Full Text :
- https://doi.org/10.12968/jowc.2009.18.6.42800