Back to Search
Start Over
Proteomic Investigation in A549 Lung Cell Line Stably Infected by HPV16E6/E7 Oncogenes.
- Source :
- Respiration; 2009, Vol. 77 Issue 4, p427-439, 13p, 1 Black and White Photograph, 1 Diagram, 3 Charts, 3 Graphs
- Publication Year :
- 2009
-
Abstract
- Background: Data have accumulated implicating the involvement of oncogenic human papillomaviruses (HPVs) in bronchial carcinogenesis. We recently described the presence of oncogenic HPV transcripts in non-small cell lung cancers. Objective: To investigate the role of oncogenic HPVs in lung carcinogenesis. Material and Methods: The lung cell line A549 stably infected with HPV16E6, HPV16E7 and HPVE6/E7 constructs was used to investigate the protein profile changes associated with the expression of these oncogenes. Replicated two-dimensional gel electrophoresis gels from uninfected and stably HPV16E6-, E7-, and E6/E7-infected A549 cells were compared for changes in protein profile. Protein identification was achieved by peptide mass fingerprinting by MALDI-TOF-MS and nLC-ESI-Q-TOF-MS/MS peptide ladder sequencing. Results: We identified 17 different polypeptides whose average normalized spot intensity was statistically significant (p < 0.05) and differed by 2-fold. Relationships between differentially expressed proteins and the HPV-induced infection mechanism have been clustered by knowledge-base database functional association network analysis. Conclusion: The impact of Hsp27, annexin III, annexin IV, Gp96 and TPT1 on the cellular response mechanism to HPV infection is presented and discussed. Copyright © 2008 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00257931
- Volume :
- 77
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Respiration
- Publication Type :
- Academic Journal
- Accession number :
- 40411804
- Full Text :
- https://doi.org/10.1159/000176209